Lifetime Imaging of the Chemical Microenvironment and Metabolic Activity in Biofilms and 3D Bioprinted Constructs
Objectives
3D mapping of the chemical microenvironment in microbial biofilms remains a major challenge to our understanding of this most common microbial lifestyle. Our team and collaborators will develop new experimental platforms for
the application of O2 sensing probes compatible with PLIM and FLIM platforms in biofilms,
the incorporation of such sensing probes within functionalized bioinks, and
realizing 3D mapping of O2 and biomass in natural and bioprinted biofilms containing phototrophic and heterotrophic microbes. Together with collaborators, we will also explore imaging of NAD(P)H and develop computational models for O2 dynamics and distribution in such structures.
Host Academic Institution: University of Copenhagen