Coordinated measurement of FLIM photonics with electrophysiological measurements and stimulation

Objectives

1. Transparent Microelectrode Arrays (tMEA) developed and commercialised by NMI (Reutlingen) capable of both stimulation and recording, will be adapted to the FLIM facility in TCD (and adapted to a design pipeline where its application to other system configurations can be achieved).

2. Experiments performed on 2D monolayer cultures and allow cell-specific electrophysiological recordings (and single cell FLIM imaging); whereby the two can be matched to an individual cell.

3. Similarly in 2D; co-culture of iPSC-derived cardiomyocytes with complimentary cell types (macrophages, endothelial cells, fibroblasts) will be performed to establish a system whereby one can uncover the impact of stimulation and cell re/de polarisation on adjacent cells.

4. Scale up to proof of concept 3D cardiomyocyte modelTo train the DC in theory of python coding and application of appropriate machine learning approaches in preparing a classification algorithm based on multiparametric input and output data.

Host Academic Institution: Trinity College Dublin