Objectives

1. Develop an isotropic resolution 3D imaging platform for fluorescence lifetime imaging (ISO-FLIM) to visualise fast molecular dynamics associated with neuronal activity within live bioengineered human pluripotent stem cell (hPSC) derived neural cultures- acquire quantitative fluorescence imaging data from live stem-cell derived neurons in 3D cultures with a massively parallel video-rate multifocal multiphoton system for fluorescence lifetime imaging employing a truly isotropic light-sheet modality.

2. With this new imaging platform we will acquisition quantitative data on calcium, voltage, ATP metabolism and any other fast-changing dynamic process that directly affects neuronal activity, within live cells through simultaneous imaging of physiologically relevant volumes.

Host Academic Institution: King’s College London